NASC Stock Detail Page
NASC ID: N571555
Name: SALK_071555
ABRC stock number: SALK_071555
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_071555.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G27090.1 (at chr 3 pos 9991068 (W/9991024-9991068) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools: P 48, R 45, C 49 N 42. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO406572. SALKseq_071555.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G19910.1 and AT4G19920.1=UTR3 (at chr 4 pos 10796963 (C/10796963-10797041) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 48, R 45, C 49 N 42. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409908. SALKseq_071555.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G15300.1 and AT4G15310.1 (at chr 4 pos 8732961 (W/8732923-8732961) on the TAIR10). The sequences' 1-39 bps mapped on the genome, 41-64 bps onto the T-DNA. Pools P 48, R 45, C 49 N 42. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO413269.
Donation Date: 2002-01-07
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_071555
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_071555C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.