NASC

NASC ID: N571555

Name: SALK_071555

ABRC stock number: SALK_071555

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_071555.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G27090.1 (at chr 3 pos 9991068 (W/9991024-9991068) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools: P 48, R 45, C 49 N 42. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO406572.

SALKseq_071555.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G19910.1 and AT4G19920.1=UTR3 (at chr 4 pos 10796963 (C/10796963-10797041) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 48, R 45, C 49 N 42. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409908.

SALKseq_071555.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G15300.1 and AT4G15310.1 (at chr 4 pos 8732961 (W/8732923-8732961) on the TAIR10). The sequences' 1-39 bps mapped on the genome, 41-64 bps onto the T-DNA. Pools P 48, R 45, C 49 N 42. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO413269.

Donation Date: 2002-01-07

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_071555

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_071555C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
		WAK3	AT3G27090 AT4G15300 AT4G15310 AT4G19910 AT4G19920 At1g21240

EMBL accession:

ED596821

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.