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The European Arabidopsis Stock Centre

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NASC ID: N575725

Name: SALK_075725

ABRC stock number: SALK_075725

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

June 2002 TAIR gene annotation - T5M7.15 R300-5'. At3g25660. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_075725.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G25660.1 (at chr 3 pos 9342217 (W/9342156-9342217) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 62, R 64, C 62 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO369869.

SALKseq_075725.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intron of AT1G52630.1 (at chr 1 pos 19608013 (C/19608013-19608048) on the TAIR10). The sequences' 1-36 bps mapped on the genome, 65-83 bps onto the T-DNA. Pools P 62, R 64, C 62 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO366525.

SALKseq_075725.101 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G46000.1 and AT5G46010.1 (at chr 5 pos 18657069 (W/18657009-18657069) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 62, R 64, C 62 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO373229.

Donation Date: 2002-01-06

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_075725

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_075725C.

Status Price (£)
Available £11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.