NASC

NASC ID: N576565

Name: SALK_076565

ABRC stock number: SALK_076565

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

June 2002 TAIR gene annotation - F1N21.22 Exon. At1g67400. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_076565.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G67400.1 (at chr 1 pos 25249431 (W/25249377-25249431) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 62, R 66, C 68 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO416694.

SALKseq_076565.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G26260.1 (at chr 4 pos 13297622 (W/13297577-13297622) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 54-72 bps onto the T-DNA. Pools P 62, R 66, C 68 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO420012.

SALKseq_076565.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G27420.1 (at chr 4 pos 13714645 (W/13714567-13714645) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 62, R 66, C 68 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO423328.

SALKseq_076565.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G28993=P355 and AT5G28996 (at chr 5 pos 11020234 (C/11020234-11020311) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 62, R 66, C 68 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO426645.

SALKseq_076565.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G09440.1=UTR3 and AT5G09445.1=P355 (at chr 5 pos 2939725 (C/2939725-2939780) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 62, R 66, C 68 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO429959.

Donation Date: 2002-01-06

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_076565

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_076565C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT4G26260 AT4G27420 AT5G09440 AT5G09445 AT5G28993 AT5G28996 At1g67400

EMBL accession:

BH857227

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.