NASC

NASC ID: N576628

Name: SALK_076628

ABRC stock number: SALK_076628

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

June 2002 TAIR gene annotation . Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_076628.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G63080.1 (at chr 3 pos 23309739 (W/23309686-23309739) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 62, R 66, C 67 N 62. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO426652.

SALKseq_076628.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G16740.1=UTR3 and AT2G16750.1=P355 (at chr 2 pos 7269971 (W/7269910-7269971) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 62, R 66, C 67 N 62. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO423335.

SALKseq_076628.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G61450.1 (at chr 5 pos 24712980 (C/24712980-24713058) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 62, R 66, C 67 N 62. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO429966.

Donation Date: 2002-01-06

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_076628

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_076628C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G16740 AT2G16750 AT5G61450 At3g63080

EMBL accession:

CC180133

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.