NASC Stock Detail Page
NASC ID: N577125
Name: SALK_077125
ABRC stock number: SALK_077125
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_077125.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G47310.1=UTR3 and AT1G47317.1 (at chr 1 pos 17345781 (W/17345705-17345781) on the TAIR10). The sequences' 1-77 bps mapped on the genome. Pools P 64, R 61, C 61 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO430042. SALKseq_077125.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G16420.1 (at chr 1 pos 5613088 (W/5613011-5613088) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools: P 64, R 61, C 61 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO433348. SALKseq_077125.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G04570.1=UTR3 and AT2G04580.1 (at chr 2 pos 1597992 (W/1597915-1597992) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 64, R 61, C 61 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO436652. SALKseq_077125.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G04570.1=UTR3 and AT2G04580.1 (at chr 2 pos 1599557 (C/1599557-1599605) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 45-70 bps onto the T-DNA. Pools P 64, R 61, C 61 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO416779. SALKseq_077125.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G42370.2 (at chr 5 pos 16939048 (C/16939048-16939107) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 54-73 bps onto the T-DNA. Pools P 64, R 61, C 61 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO420095.
Donation Date: 2002-01-07
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_077125
Part of : SALK lines
Stock type: individual line
Material type: seed
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.