Home | About NASC | Address/Staff
Ask a question | Help

NASC

The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N577125

Name: SALK_077125

ABRC stock number: SALK_077125

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_077125.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G47310.1=UTR3 and AT1G47317.1 (at chr 1 pos 17345781 (W/17345705-17345781) on the TAIR10). The sequences' 1-77 bps mapped on the genome. Pools P 64, R 61, C 61 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO430042.

SALKseq_077125.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G16420.1 (at chr 1 pos 5613088 (W/5613011-5613088) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools: P 64, R 61, C 61 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO433348.

SALKseq_077125.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G04570.1=UTR3 and AT2G04580.1 (at chr 2 pos 1597992 (W/1597915-1597992) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 64, R 61, C 61 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO436652.

SALKseq_077125.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G04570.1=UTR3 and AT2G04580.1 (at chr 2 pos 1599557 (C/1599557-1599605) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 45-70 bps onto the T-DNA. Pools P 64, R 61, C 61 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO416779.

SALKseq_077125.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G42370.2 (at chr 5 pos 16939048 (C/16939048-16939107) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 54-73 bps onto the T-DNA. Pools P 64, R 61, C 61 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO420095.

Donation Date: 2002-01-07

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_077125

Part of : SALK lines

Stock type: individual line

Material type: seed

Status Price (£)
Available £11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.