NASC

NASC ID: N577420

Name: SALK_077420

ABRC stock number: SALK_077420

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

June 2002 TAIR gene annotation - F11M15.19 R300-5'. At1g51330. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_077420.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G51330.1 and AT1G51340.2=P355 (at chr 1 pos 19029913 (W/19029873-19029913) on the TAIR10). The sequences' 1-41 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 57, R 60, C 58 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO379961.

SALKseq_077420.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G10570.1 (at chr 1 pos 3489582 (W/3489544-3489582) on the TAIR10). The sequences' 1-39 bps mapped on the genome, 49-79 bps onto the T-DNA. Pools P 57, R 60, C 58 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO383306.

SALKseq_077420.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G26760.1 (at chr 5 pos 9407505 (C/9407505-9407583) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 57, R 60, C 58 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO386653.

Donation Date: 2002-01-06

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_077420

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_077420C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G10570 AT1G51340 AT5G26760 At1g51330

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.