NASC

NASC ID: N578330

Name: SALK_078330

ABRC stock number: SALK_078330

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

July 2002 TAIR gene annotation - At5g47660. MNJ7.25 Intron. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_078330.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G47660.1 (at chr 5 pos 19313275 (W/19313198-19313275) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 63, R 69, C 68 N 62. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO420266.

SALKseq_078330.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G32190.2=P355 and AT1G32200.1=UTR3 (at chr 1 pos 11600920 (W/11600858-11600920) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 63, R 69, C 68 N 62. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO433519.

SALKseq_078330.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G72710.1 (at chr 1 pos 27373247 (W/27373211-27373247) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 37-66 bps onto the T-DNA. Pools P 63, R 69, C 68 N 62. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO436822.

SALKseq_078330.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G63070.1 (at chr 3 pos 23309182 (W/23309123-23309182) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 52-79 bps onto the T-DNA. Pools P 63, R 69, C 68 N 62. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO416950.

SALKseq_078330.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G65500.1 and AT5G65510.1 (at chr 5 pos 26185525 (W/26185448-26185525) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 63, R 69, C 68 N 62. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO423582.

Donation Date: 2002-01-07

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_078330

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_078330C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G32190 AT1G32200 AT1G72710 AT3G63070 AT5G65500 AT5G65510 At5g47660

EMBL accession:

BH853823

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.