NASC Stock Detail Page
NASC ID: N579216
Name: SALK_079216
ABRC stock number: SALK_079216
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. July 2002 TAIR gene annotation - At1g71990. F17M19.14 L300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_079216.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G71990.1 (at chr 1 pos 27100101 (C/27100101-27100164) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 79-100 bps onto the T-DNA. Pools P 66, R 70, C 66 N 62. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO436989. SALKseq_079216.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G79310.1 (at chr 1 pos 29833849 (W/29833772-29833849) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 66, R 70, C 66 N 62. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO417117. SALKseq_079216.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G30640.1 (at chr 4 pos 14953515 (W/14953438-14953515) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 66, R 70, C 66 N 62. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO420433. SALKseq_079216.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G01445.1 (at chr 5 pos 182225 (C/182225-182306) on the TAIR10). The sequences' 1-82 bps mapped on the genome, 83-100 bps onto the T-DNA. Pools P 66, R 70, C 66 N 62. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO423749.
Donation Date: 2002-01-07
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_079216
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_079216C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.