NASC Stock Detail Page
NASC ID: N579519
Name: SALK_079519
ABRC stock number: SALK_079519
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_079519.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G48340.1 (at chr 3 pos 17898579 (W/17898522-17898579) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 65, R 64, C 67 N 64. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO427108. SALKseq_079519.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G63090.1=P355 and AT1G63100.1 (at chr 1 pos 23394702 (W/23394642-23394702) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 65, R 64, C 67 N 64. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO423792. SALKseq_079519.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G52480.1 (at chr 5 pos 21297115 (W/21297081-21297115) on the TAIR10). The sequences' 1-35 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 65, R 64, C 67 N 64. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO430424. SALKseq_079519.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G53620.3=UTR3 and AT5G53635.1 (at chr 5 pos 21785576 (C/21785576-21785637) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 65, R 64, C 67 N 64. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO433729. SALKseq_079519.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G58780.1 (at chr 5 pos 23736658 (C/23736658-23736735) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 65, R 64, C 67 N 64. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO437033.
Donation Date: 2002-01-07
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_079519
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_079519C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
|---|---|---|---|
|
|
|
|
Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.