NASC Stock Detail Page
NASC ID: N580387
Name: SALK_080387
ABRC stock number: SALK_080387
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_080387.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G10470.2 (at chr 5 pos 3297631 (W/3297588-3297631) on the TAIR10). The sequences' 1-44 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 68, R 64, C 63 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO423904. SALKseq_080387.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G30330.2=P355 and AT1G30340.1=P355 (at chr 1 pos 10695994 (W/10695931-10695994) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 68, R 64, C 63 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO433840. SALKseq_080387.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G55270.1 (at chr 3 pos 20496045 (W/20495997-20496045) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 68, R 64, C 63 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO437144. SALKseq_080387.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G16620.1 (at chr 4 pos 9360406 (W/9360329-9360406) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 68, R 64, C 63 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO417272. SALKseq_080387.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G57130.1 (at chr 5 pos 23147056 (W/23147007-23147056) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 68, R 64, C 63 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO420588.
Donation Date: 2003-04-07
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_080387
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_080387C.
| Status | Price (£) |
|---|---|
| Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.