NASC Stock Detail Page
NASC ID: N582006
Name: SALK_082006
ABRC stock number: SALK_082006
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. February 2003 TAIR gene annotation - At3g24780 K7P8.7 Ex on K7P8.7 Ex on. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_082006.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G24780.1 (at chr 3 pos 9050794 (W/9050762-9050794) on the TAIR10). The sequences' 1-33 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 71, R 79, C 80 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO427518. SALKseq_082006.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G04470.1=P355 and AT1G04480.1=P355 (at chr 1 pos 1215647 (C/1215647-1215685) on the TAIR10). The sequences' 1-39 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 71, R 79, C 80 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO417570. SALKseq_082006.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G48040.1 (at chr 1 pos 17722058 (W/17721998-17722058) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 71, R 79, C 80 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO420887. SALKseq_082006.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G37360.1=P355 and AT2G37370.1=P355 (at chr 2 pos 15677088 (C/15677088-15677146) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 71, R 79, C 80 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO424203.
Donation Date: 2003-03-11
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_082006
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_082006C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.