NASC

NASC ID: N582378

Name: SALK_082378

ABRC stock number: SALK_082378

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_082378.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G16290.1 (at chr 5 pos 5333770 (C/5333770-5333827) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 70, R 70, C 64 N 69. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO427590.

SALKseq_082378.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G18690.2=UTR3 and AT2G18700.1 (at chr 2 pos 8101728 (W/8101688-8101728) on the TAIR10). The sequences' 1-41 bps mapped on the genome, 52-79 bps onto the T-DNA. Pools P 70, R 70, C 64 N 69. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO420959.

SALKseq_082378.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G18690.2=UTR3 and AT2G18700.1 (at chr 2 pos 8101730 (C/8101730-8101774) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 70, R 70, C 64 N 69. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO424275.

SALKseq_082378.5 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G19090.1 (at chr 5 pos 6387578 (W/6387519-6387578) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 70, R 70, C 64 N 69. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO430907.

SALKseq_082378.6 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G23700.1 (at chr 5 pos 7995884 (W/7995824-7995884) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 70, R 70, C 64 N 69. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO434212.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_082378

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_082378C.

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G18690 AT2G18700 At5g16290 AT5G19090 AT5G23700

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.