NASC

NASC ID: N583327

Name: SALK_083327

ABRC stock number: SALK_083327

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

July 2002 TAIR gene annotation - At1g26290. F28B23.5 L300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_083327.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G26300.1 (at chr 1 pos 9097864 (W/9097820-9097864) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 73, R 77, C 72 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO421132.

SALKseq_083327.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G34150.1=P355 and AT2G34160.1=P355 (at chr 2 pos 14425319 (C/14425319-14425379) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 73, R 77, C 72 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO424448.

SALKseq_083327.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G00990.1 (at chr 4 pos 431076 (W/431019-431076) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 73, R 77, C 72 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO427763.

SALKseq_083327.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G65630.1=P355 and AT5G65640.1=P355 (at chr 5 pos 26230046 (W/26229988-26230046) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 73, R 77, C 72 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431080.

Donation Date: 2002-01-07

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_083327

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_083327C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			At1g26300 AT1G26300 AT2G34150 AT2G34160 AT4G00990 AT5G65630 AT5G65640 At1g26290

EMBL accession:

BH856137

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.