NASC

NASC ID: N584096

Name: SALK_084096

ABRC stock number: SALK_084096

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

December 2002 TAIR gene annotation - At1g65070 F16G16.7 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_084096.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G59890.1 (at chr 3 pos 22125118 (C/22125118-22125178) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 72, R 77, C 76 N 73. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO437832.

SALKseq_084096.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G21120.1 (at chr 4 pos 11269799 (W/11269734-11269799) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 72, R 77, C 76 N 73. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO417959.

SALKseq_084096.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G32280.1 (at chr 4 pos 15583746 (C/15583746-15583818) on the TAIR10). The sequences' 1-73 bps mapped on the genome, 73-95 bps onto the T-DNA. Pools P 72, R 77, C 76 N 73. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO421275.

SALKseq_084096.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G06474.1 (at chr 4 pos 3051826 (W/3051759-3051826) on the TAIR10). The sequences' 1-68 bps mapped on the genome, 56-95 bps onto the T-DNA. Pools P 72, R 77, C 76 N 73. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO424592.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_084096

Part of : SALK lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			At1g65080 AT3G59890 AT4G06474 AT4G21120 AT4G32280 At1g65070

EMBL accession:

BZ594463

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.