NASC Stock Detail Page
NASC ID: N584797
Name: SALK_084797
ABRC stock number: SALK_084797
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. July 2002 TAIR gene annotation - At5g50240. K6A12.10 L300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_084797.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G50240.1 (at chr 5 pos 20451175 (W/20451115-20451175) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 70, R 69, C 66 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO437974. SALKseq_084797.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G10040.1 (at chr 4 pos 6276724 (C/6276724-6276802) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 70, R 69, C 66 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO434670. SALKseq_084797.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G22110.1 (at chr 5 pos 7330961 (C/7330961-7331039) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 70, R 69, C 66 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO418101.
Donation Date: 2002-01-07
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_084797
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_084797C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.