NASC Stock Detail Page
NASC ID: N584988
Name: SALK_084988
ABRC stock number: SALK_084988
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. July 2002 TAIR gene annotation - At3g30405. MVA11.8 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_084988.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G30405.1 (at chr 3 pos 12024032 (C/12024032-12024074) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 52-78 bps onto the T-DNA. Pools P 71, R 80, C 74 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431399. SALKseq_084988.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G62740.1 (at chr 1 pos 23231003 (W/23230954-23231003) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 71, R 80, C 74 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO424769. SALKseq_084988.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G21040.1 and AT2G21045.1=P355 (at chr 2 pos 9027349 (W/9027289-9027349) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 71, R 80, C 74 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO428084. SALKseq_084988.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G21090.1=P355 and AT3G21100.2=P355 (at chr 3 pos 7396307 (W/7396263-7396307) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 71, R 80, C 74 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO434706. SALKseq_084988.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G34970.1 (at chr 4 pos 16654397 (W/16654344-16654397) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 71, R 80, C 74 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438010.
Donation Date: 2002-01-07
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_084988
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_084988C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.