NASC

NASC ID: N585156

Name: SALK_085156

ABRC stock number: SALK_085156

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

December 2002 TAIR gene annotation - At5g18620 T1A4.2 R300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_085156.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G18620.1 (at chr 5 pos 6199134 (C/6199134-6199200) on the TAIR10). The sequences' 2-68 bps mapped on the genome, 58-95 bps onto the T-DNA. Pools P 71, R 74, C 78 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO424802.

SALKseq_085156.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT2G45700.1 (at chr 2 pos 18827190 (W/18827136-18827190) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 71, R 74, C 78 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO418169.

SALKseq_085156.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G59420.1 (at chr 5 pos 23962617 (W/23962540-23962617) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 71, R 74, C 78 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO421485.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_085156

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_085156C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Ploidy: 2(n)

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G45700 AT5G59420 At5g18620

EMBL accession:

BZ594742

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.