NASC

NASC ID: N585205

Name: SALK_085205

ABRC stock number: SALK_085205

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

December 2002 TAIR gene annotation - At2g29190 F16P2.43 R300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_085205.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G29190.1 (at chr 2 pos 12548089 (W/12548027-12548089) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 68, R 69, C 63 N 67. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO424812.

SALKseq_085205.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G80360.1=P355 and AT1G80370.1=P355 (at chr 1 pos 30213232 (W/30213172-30213232) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 68, R 69, C 63 N 67. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO421495.

SALKseq_085205.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G34950.1=P355 and AT4G34960.1=P355 (at chr 4 pos 16647676 (W/16647622-16647676) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 68, R 69, C 63 N 67. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO428127.

SALKseq_085205.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G15790.1 (at chr 4 pos 8982424 (W/8982364-8982424) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 68, R 69, C 63 N 67. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431442.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_085205

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_085205C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G80360 AT1G80370 AT4G15790 AT4G34950 AT4G34960 At2g29190

EMBL accession:

BZ594782

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.