NASC Stock Detail Page
NASC ID: N585428
Name: SALK_085428
ABRC stock number: SALK_085428
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. December 2002 TAIR gene annotation - At5g57330 MJB24.14 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_085428.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G68880.1=P355 and AT1G68890.1=P355 (at chr 1 pos 25896164 (C/25896164-25896217) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 77, R 74, C 77 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO428169. SALKseq_085428.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G28300.1 (at chr 1 pos 9897502 (C/9897502-9897557) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 77, R 74, C 77 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431484. SALKseq_085428.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G57330.1=UTR3 and AT5G57340.1=P355 (at chr 5 pos 23222987 (C/23222987-23223050) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 77, R 74, C 77 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO434791. SALKseq_085428.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G64360.1 (at chr 5 pos 25737838 (W/25737796-25737838) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 77, R 74, C 77 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438095.
Donation Date: 2003-01-30
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_085428
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_085428C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.