NASC

NASC ID: N587439

Name: SALK_087439

ABRC stock number: SALK_087439

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

August 2002 TAIR gene annotation - T27A16.20 L300-5', At2g29700. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_087439.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G29700.1 (at chr 2 pos 12697398 (W/12697359-12697398) on the TAIR10). The sequences' 1-40 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 76, R 72, C 80 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO428545.

SALKseq_087439.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G30130.2=UTR3 and AT1G30135.1=P355 (at chr 1 pos 10595880 (W/10595803-10595880) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 76, R 72, C 80 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO425231.

SALKseq_087439.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G18720.1 (at chr 2 pos 8116337 (W/8116260-8116337) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools: P 76, R 72, C 80 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431861.

Donation Date: 2002-01-09

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_087439

Part of : SALK lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G30130 AT1G30135 AT2G18720 At2g29700

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.