NASC

NASC ID: N588163

Name: SALK_088163

ABRC stock number: SALK_088163

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

August 2002 TAIR gene annotation - T24H18.120 L300-5', At5g12950. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_088163.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G12950.1 (at chr 5 pos 4092971 (C/4092971-4093020) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 51-78 bps onto the T-DNA. Pools P 75, R 79, C 77 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435302.

SALKseq_088163.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G09850.1 (at chr 1 pos 3203273 (C/3203273-3203327) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 51-78 bps onto the T-DNA. Pools P 75, R 79, C 77 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO428679.

SALKseq_088163.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT2G20740.1 (at chr 2 pos 8937428 (W/8937392-8937428) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 51-78 bps onto the T-DNA. Pools P 75, R 79, C 77 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431995.

Donation Date: 2002-01-09

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_088163

Part of : SALK lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G09850 AT2G20740 At5g12950

EMBL accession:

ED597871

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.