NASC Stock Detail Page
NASC ID: N588174
Name: SALK_088174
ABRC stock number: SALK_088174
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. August 2002 TAIR gene annotation - T1O16.5 R300-5', At2g14360. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_088174.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G14350.1 (at chr 2 pos 6082438 (W/6082384-6082438) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 75, R 79, C 76 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435304. SALKseq_088174.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G22910.1 (at chr 3 pos 8117967 (W/8117922-8117967) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 75, R 79, C 76 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438608. SALKseq_088174.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G53900.1 (at chr 5 pos 21883404 (W/21883355-21883404) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 52-78 bps onto the T-DNA. Pools P 75, R 79, C 76 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO418735. SALKseq_088174.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G66160.1 (at chr 5 pos 26446968 (W/26446917-26446968) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 75, R 79, C 76 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO422051.
Donation Date: 2002-01-09
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_088174
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_088174C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.