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The European Arabidopsis Stock Centre

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NASC ID: N588470

Name: SALK_088470

ABRC stock number: SALK_088470

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_088470.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G55940.1 and AT3G55950.1=UTR3 (at chr 3 pos 20752723 (W/20752647-20752723) on the TAIR10). The sequences' 1-77 bps mapped on the genome. Pools P 76, R 80, C 77 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO425422.

SALKseq_088470.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT2G38440.1 (at chr 2 pos 16095372 (W/16095335-16095372) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 42-78 bps onto the T-DNA. Pools P 76, R 80, C 77 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO422105.

SALKseq_088470.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G15490.1 (at chr 3 pos 5229442 (C/5229442-5229507) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 76, R 80, C 77 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO428736.

SALKseq_088470.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G24030.1 (at chr 4 pos 12486553 (C/12486553-12486630) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 76, R 80, C 77 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO432052.

SALKseq_088470.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G27495.1 (at chr 5 pos 9706720 (W/9706664-9706720) on the TAIR10). The sequences' 4-60 bps mapped on the genome, 54-78 bps onto the T-DNA. Pools: P 76, R 80, C 77 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435359.

Donation Date: 2002-01-09

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_088470

Part of : SALK lines

Stock type: individual line

Material type: seed

Status Price (£)
Available £11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.