NASC

NASC ID: N588882

Name: SALK_088882

ABRC stock number: SALK_088882

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

August 2002 TAIR gene annotation - T8P19.110 R300-5' At3g48610 T8P19.120 L300-3', At3g48600. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_088882.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G48600.1 (at chr 3 pos 18011565 (W/18011510-18011565) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 77, R 80, C 78 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO425498.

SALKseq_088882.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G12610.1=UTR3 and AT4G12617.1 (at chr 4 pos 7459141 (W/7459084-7459141) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 77, R 80, C 78 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO428812.

SALKseq_088882.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G57360.1 (at chr 5 pos 23241239 (W/23241180-23241239) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 53-78 bps onto the T-DNA. Pools P 77, R 80, C 78 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO432128.

SALKseq_088882.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G10504.1 and AT5G10510.2=P355 (at chr 5 pos 3311823 (W/3311765-3311823) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 77, R 80, C 78 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435435.

Donation Date: 2002-01-09

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_088882

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N663251 (SALK_088882C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			At3g48600 AT3G48600 AT4G12610 AT4G12617 AT5G10504 AT5G10510 AT5G57360 At3g48610

EMBL accession:

ED598184

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.