NASC

NASC ID: N589260

Name: SALK_089260

ABRC stock number: SALK_089260

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

August 2002 TAIR gene annotation - K10A8.20 Exon, At5g17540. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_089260.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G42885.1 (at chr 2 pos 17846320 (W/17846260-17846320) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools: P 2, R 8, C 4 N 5. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO432197.

SALKseq_089260.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G02030.1 (at chr 2 pos 482360 (C/482360-482409) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 2, R 8, C 4 N 5. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435504.

SALKseq_089260.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G39710.1 (at chr 4 pos 18428357 (C/18428357-18428417) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 2, R 8, C 4 N 5. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438807.

Donation Date: 2002-01-09

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_089260

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_089260C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G02030 AT2G42885 AT4G39710 At5g17540

EMBL accession:

ED598297

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.