NASC

NASC ID: N590261

Name: SALK_090261

ABRC stock number: SALK_090261

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

August 2002 TAIR gene annotation - MGO3.8 Intron, At5g60100. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_090261.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G60100.1 (at chr 5 pos 24198297 (C/24198297-24198338) on the TAIR10). The sequences' 1-42 bps mapped on the genome, 44-78 bps onto the T-DNA. Pools P 77, R 71, C 76 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO422422.

SALKseq_090261.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G34750.1 (at chr 1 pos 12738209 (W/12738143-12738209) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 77, R 71, C 76 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435674.

SALKseq_090261.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G67090.2=P355 and AT1G67100.1=P355 (at chr 1 pos 25052747 (W/25052691-25052747) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 77, R 71, C 76 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438978.

SALKseq_090261.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G04450.1 (at chr 4 pos 2218878 (C/2218878-2218941) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 77, R 71, C 76 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO419106.

Donation Date: 2002-01-09

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_090261

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_090261C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G34750 AT1G67090 AT1G67100 AT4G04450 At5g60100

EMBL accession:

ED598696

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.