NASC

NASC ID: N593640

Name: SALK_093640

ABRC stock number: SALK_093640

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_093640.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G09100.2 (at chr 3 pos 2789999 (C/2789999-2790060) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 79, R 72, C 78 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO422835.

SALKseq_093640.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G52760.1=UTR3 and AT1G52770.1=P355 (at chr 1 pos 19654475 (C/19654475-19654515) on the TAIR10). The sequences' 1-41 bps mapped on the genome, 51-77 bps onto the T-DNA. Pools P 79, R 72, C 78 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO439388.

SALKseq_093640.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G11980 and AT2G11983=P355 (at chr 2 pos 4829969 (W/4829892-4829969) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 79, R 72, C 78 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO419517.

SALKseq_093640.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G25870.1=P355 and AT3G25880.1 (at chr 3 pos 9469170 (C/9469170-9469208) on the TAIR10). The sequences' 1-39 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 79, R 72, C 78 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO426152.

Donation Date: 2002-01-09

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_093640

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_093640C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G52760 AT1G52770 AT2G11980 AT2G11983 AT3G25870 AT3G25880 At3g09100

EMBL accession:

CC055304

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.