NASC

NASC ID: N594118

Name: SALK_094118

ABRC stock number: SALK_094118

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_094118.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G22970.2=UTR3 and AT3G22980.1=UTR3 (at chr 3 pos 8159864 (C/8159864-8159926) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 80, R 80, C 76 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO419555.

SALKseq_094118.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G53210.1 (at chr 3 pos 19721317 (C/19721317-19721398) on the TAIR10). The sequences' 2-82 bps mapped on the genome, 77-98 bps onto the T-DNA. Pools P 80, R 80, C 76 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO439425.

SALKseq_094118.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G19038.1 (at chr 4 pos 10430216 (W/10430176-10430216) on the TAIR10). The sequences' 1-41 bps mapped on the genome, 42-63 bps onto the T-DNA. Pools P 80, R 80, C 76 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO422873.

Donation Date: 2002-01-09

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_094118

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_094118C.

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G22970 AT3G22980 At3g53210 AT4G19038

EMBL accession:

CC456224

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.