NASC

NASC ID: N594207

Name: SALK_094207

ABRC stock number: SALK_094207

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

August 2002 TAIR gene annotation - T30C3.90 Intron, At4g25420. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_094207.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G25420.1 (at chr 4 pos 12991691 (C/12991691-12991747) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 53-78 bps onto the T-DNA. Pools P 77, R 78, C 76 N 71. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO429513.

SALKseq_094207.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G31840.2=UTR3 and AT1G31850.1=P355 (at chr 1 pos 11427452 (W/11427399-11427452) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 53-78 bps onto the T-DNA. Pools P 77, R 78, C 76 N 71. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO426200.

SALKseq_094207.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G26220.1=P355 and AT5G26230.1 (at chr 5 pos 9172984 (C/9172984-9173037) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 77, R 78, C 76 N 71. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO432826.

Donation Date: 2002-01-09

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_094207

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_094207C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G31840 AT1G31850 AT5G26220 AT5G26230 At4g25420

EMBL accession:

ED599831

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.