NASC

NASC ID: N594255

Name: SALK_094255

ABRC stock number: SALK_094255

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

August 2002 TAIR gene annotation - T8L23.13 Exon, At1g57660. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_094255.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G57660.1 (at chr 1 pos 21356261 (W/21356212-21356261) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 51-78 bps onto the T-DNA. Pools P 80, R 80, C 71 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO436145.

SALKseq_094255.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G56950.2=P355 and AT3G56960.1 (at chr 3 pos 21080866 (C/21080866-21080942) on the TAIR10). The sequences' 1-77 bps mapped on the genome, 68-95 bps onto the T-DNA. Pools P 80, R 80, C 71 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO439445.

SALKseq_094255.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G63370.1 (at chr 3 pos 23402045 (W/23401985-23402045) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 80, R 80, C 71 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO419575.

Donation Date: 2002-01-09

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_094255

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N663377 (SALK_094255C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G56950 AT3G56960 AT3G63370 At1g57660

EMBL accession:

ED599859

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.