NASC Stock Detail Page
NASC ID: N599598
Name: SALK_099598
ABRC stock number: SALK_099598
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_099598.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G60160.1=P355 and AT3G60164 (at chr 3 pos 22229951 (C/22229951-22230029) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 10, R 9, C 5 N 7. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO429593. SALKseq_099598.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G60966.1=UTR3 and AT3G60970.1 (at chr 3 pos 22555333 (W/22555280-22555333) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 10, R 9, C 5 N 7. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO432906. SALKseq_099598.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G04650.1 (at chr 5 pos 1334241 (C/1334241-1334303) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 10, R 9, C 5 N 7. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO436215. SALKseq_099598.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G61440.1 (at chr 5 pos 24707536 (C/24707536-24707614) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 10, R 9, C 5 N 7. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO439516. SALKseq_099598.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G23870.2 (at chr 5 pos 8048939 (W/8048893-8048939) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 10, R 9, C 5 N 7. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO419643.
Donation Date: 2002-01-10
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_099598
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_099598C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.