NASC

NASC ID: N600504

Name: SALK_100504

ABRC stock number: SALK_100504

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At2g15920 F19G14. 8 Exon F19G14. 8 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_100504.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G36950.1=P355 and AT4G36960.1=P355 (at chr 4 pos 17426100 (W/17426058-17426100) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 52-78 bps onto the T-DNA. Pools P 6, R 3, C 6 N 9. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO422986.

SALKseq_100504.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G01380.1 (at chr 5 pos 156818 (W/156762-156818) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 6, R 3, C 6 N 9. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO426307.

SALKseq_100504.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G51550.1=P355 and AT5G51560.1=P355 (at chr 5 pos 20944616 (C/20944616-20944676) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 6, R 3, C 6 N 9. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO429617.

SALKseq_100504.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G03650.1 (at chr 5 pos 933953 (W/933908-933953) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 53-79 bps onto the T-DNA. Pools P 6, R 3, C 6 N 9. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO432931.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_100504

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_100504C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT4G36950 AT4G36960 AT5G01380 AT5G03650 AT5G51550 AT5G51560 At2g15920

EMBL accession:

BZ597217

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.