NASC

NASC ID: N601867

Name: SALK_101867

ABRC stock number: SALK_101867

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_101867.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G25640.2 (at chr 4 pos 13077009 (C/13077009-13077069) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 12, R 18, C 11 N 20. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO429647.

SALKseq_101867.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G15900.1 and AT5G15910.1=P355 (at chr 5 pos 5192282 (C/5192282-5192334) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 51-79 bps onto the T-DNA. Pools P 12, R 18, C 11 N 20. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO432958.

SALKseq_101867.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G22794.1=UTR3 and AT5G22800.1=UTR3 (at chr 5 pos 7614321 (W/7614265-7614321) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 12, R 18, C 11 N 20. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO436268.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_101867

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_101867C.

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT4G25640 AT5G15900 AT5G15910 AT5G22794 AT5G22800 At2g24310

EMBL accession:

BZ762366

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.