NASC Stock Detail Page
NASC ID: N610873
Name: SALK_110873
ABRC stock number: SALK_110873
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. January 2003 TAIR gene annotation - At1g60140 T13D8.4 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_110873.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G52350 (at chr 1 pos 19498821 (C/19498821-19498898) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 21, R 22, C 30 N 27. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO376664.
SALKseq_110873.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G77750.1=P355 and AT1G77760.1=UTR3 (at chr 1 pos 29232871 (W/29232827-29232871) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 44-70 bps onto the T-DNA. Pools P 21, R 22, C 30 N 27. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO380012.
Donation Date: 2003-01-31
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_110873
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N663657 (SALK_110873C).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.