NASC Stock Detail Page
NASC ID: N610891
Name: SALK_110891
ABRC stock number: SALK_110891
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_110891.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G70650.1 (at chr 1 pos 26639288 (W/26639232-26639288) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 21, R 29, C 30 N 21. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO406755. SALKseq_110891.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G23510.1 and AT2G23520.1=UTR3 (at chr 2 pos 10013802 (W/10013744-10013802) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 21, R 29, C 30 N 21. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO410092. SALKseq_110891.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G38780.1=UTR3 and AT5G38790.1 (at chr 5 pos 15533379 (W/15533326-15533379) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 52-78 bps onto the T-DNA. Pools P 21, R 29, C 30 N 21. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO413452.
Donation Date: 2003-01-31
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_110891
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_110891C.
Status | Price (£) |
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Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.