NASC

NASC ID: N610901

Name: SALK_110901

ABRC stock number: SALK_110901

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At4g26940 F10M23.280 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_110901.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G19620.1 (at chr 2 pos 8488843 (C/8488843-8488912) on the TAIR10). The sequences' 1-70 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 21, R 29, C 28 N 30. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO386706.

SALKseq_110901.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G16460.1 (at chr 4 pos 9285406 (C/9285406-9285486) on the TAIR10). The sequences' 1-81 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools P 21, R 29, C 28 N 30. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO390046.

SALKseq_110901.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G50140.1 and AT5G50150.1=UTR3 (at chr 5 pos 20399441 (C/20399441-20399535) on the TAIR10). The sequences' 1-95 bps mapped on the genome. Pools P 21, R 29, C 28 N 30. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO393402.

Donation Date: 2003-01-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_110901

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N663659 (SALK_110901C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G19620 AT4G16460 AT5G50140 AT5G50150 At4g26940

EMBL accession:

BZ665223

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.