NASC

NASC ID: N621565

Name: SALK_121565

ABRC stock number: SALK_121565

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_121565.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G13740.1 (at chr 5 pos 4436317 (W/4436273-4436317) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 46-69 bps onto the T-DNA. Pools P 53, R 52, C 59 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO356630.

SALKseq_121565.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G13470.1 (at chr 5 pos 4319703 (W/4319661-4319703) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 46-79 bps onto the T-DNA. Pools: P 53, R 52, C 59 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO353312.

SALKseq_121565.101 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G15070.1 (at chr 5 pos 4884807 (W/4884772-4884807) on the TAIR10). The sequences' 1-36 bps mapped on the genome, 47-65 bps onto the T-DNA. Pools P 53, R 52, C 59 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO359980.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_121565

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N653458 (SALK_121565C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT5G13470 At5g13740 AT5G15070

EMBL accession:

CC458661

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.