NASC

NASC ID: N622220

Name: SALK_122220

ABRC stock number: SALK_122220

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_122220.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G53708.1 (at chr 1 pos 20052947 (C/20052947-20053017) on the TAIR10). The sequences' 1-71 bps mapped on the genome, 72-100 bps onto the T-DNA. Pools: P 55, R 57, C 58 N 56. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO390136.

SALKseq_122220.001 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G18220.1=UTR3 and AT3G18230.1=P355 (at chr 3 pos 6250577 (C/6250577-6250637) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 55, R 57, C 58 N 56. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO396826.

SALKseq_122220.1 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G44745.1 (at chr 2 pos 18448795 (W/18448723-18448795) on the TAIR10). The sequences' 1-73 bps mapped on the genome, 70-91 bps onto the T-DNA. Pools P 55, R 57, C 58 N 56. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO393492.

Donation Date: 2002-11-01

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_122220

Part of : SALK lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G53708 AT2G44745 AT3G18220 AT3G18230

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.