NASC

NASC ID: N623052

Name: SALK_123052

ABRC stock number: SALK_123052

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At2g47630 F17A22.2 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_123052.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G47630.1 (at chr 2 pos 19535002 (W/19534924-19535002) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 53, R 60, C 58 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO393524.

SALKseq_123052.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G06437 (at chr 3 pos 1972617 (C/1972617-1972716) on the TAIR10). The sequences' 1-100 bps mapped on the genome. Pools P 53, R 60, C 58 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO396861.

SALKseq_123052.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G36915=P355 and AT1G36920.1=P355 (at chr 1 pos 13980882 (C/13980882-13980960) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 53, R 60, C 58 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO390170.

SALKseq_123052.201 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G22660.1 (at chr 3 pos 8017651 (C/8017651-8017750) on the TAIR10). The sequences' 1-100 bps mapped on the genome. Pools P 53, R 60, C 58 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400205.

Donation Date: 2003-01-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_123052

Part of : SALK lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G36915 AT1G36920 AT3G06437 AT3G22660 At2g47630

EMBL accession:

BZ292077

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.