NASC Stock Detail Page
NASC ID: N624947
Name: SALK_124947
ABRC stock number: SALK_124947
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. January 2003 TAIR gene annotation - At4g04407 T19B17.18 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_124947.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G04407.1 (at chr 4 pos 2174796 (W/2174738-2174796) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 53, R 58, C 56 N 52. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO353466. SALKseq_124947.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G16390.1=UTR3 and AT3G16400.1=P355 (at chr 3 pos 5564857 (W/5564819-5564857) on the TAIR10). The sequences' 1-39 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 53, R 58, C 56 N 52. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO350117. SALKseq_124947.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G36240.1 and AT5G36250.1=P355 (at chr 5 pos 14278960 (C/14278960-14279021) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 53, R 58, C 56 N 52. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO356791. SALKseq_124947.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G37473.1 and AT5G37474.1 (at chr 5 pos 14871921 (C/14871921-14871961) on the TAIR10). The sequences' 1-41 bps mapped on the genome, 45-79 bps onto the T-DNA. Pools P 53, R 58, C 56 N 52. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO360141.
Donation Date: 2003-01-31
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_124947
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_124947C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.