NASC

NASC ID: N625484

Name: SALK_125484

ABRC stock number: SALK_125484

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At1g04810 F13M7.20 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_125484.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G04810.1 (at chr 1 pos 1351772 (W/1351694-1351772) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 55, R 58, C 56 N 59. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO407012.

SALKseq_125484.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G66590.2=UTR3 and AT1G66600.1=P355 (at chr 1 pos 24842555 (W/24842497-24842555) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 55-79 bps onto the T-DNA. Pools P 55, R 58, C 56 N 59. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO410350.

SALKseq_125484.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G35630.1 (at chr 4 pos 16903942 (W/16903885-16903942) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 55, R 58, C 56 N 59. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO413706.

Donation Date: 2003-01-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_125484

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_125484C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G66590 AT1G66600 AT4G35630 At1g04810

EMBL accession:

BZ354613

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.