NASC

NASC ID: N625521

Name: SALK_125521

ABRC stock number: SALK_125521

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At1g75000 F25A4.4 R300-3' At1g75010 F25A4.3 L300-3' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_125521.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G28240.1=P355 and AT4G28250.1=UTR3 (at chr 4 pos 13999114 (C/13999114-13999147) on the TAIR10). The sequences' 1-34 bps mapped on the genome, 52-79 bps onto the T-DNA. Pools P 56, R 58, C 56 N 56. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO373575.
SALKseq_125521.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G10440.1 (at chr 4 pos 6461047 (C/6461047-6461103) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools: P 56, R 58, C 56 N 56. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO376922.

Donation Date: 2003-01-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_125521

Part of : SALK lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT4G10440 AT4G28240 AT4G28250 At1g75000

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.