NASC Stock Detail Page
NASC ID: N625584
Name: SALK_125584
ABRC stock number: SALK_125584
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. January 2003 TAIR gene annotation - At5g47490 MNJ7.8 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_125584.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G47490.1 (at chr 5 pos 19270079 (W/19270001-19270079) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 59, R 54, C 51 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO353501.
SALKseq_125584.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT1G16858.1 (at chr 1 pos 5770597 (W/5770536-5770597) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 59, R 54, C 51 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO350152.
Donation Date: 2003-01-31
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_125584
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_125584C.
| Status | Price (£) |
|---|---|
| Available | £10.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.