NASC Stock Detail Page
NASC ID: N626070
Name: SALK_126070
ABRC stock number: SALK_126070
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. January 2003 TAIR gene annotation - At4g23950 T32A16.120 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_126070.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G23950.1 (at chr 4 pos 12443543 (W/12443506-12443543) on the TAIR10). The sequences' 1-38 bps mapped on the genome, 55-79 bps onto the T-DNA. Pools P 56, R 51, C 51 N 57. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO360202. SALKseq_126070.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G01940.1=P355 and AT4G01950.1=UTR3 (at chr 4 pos 844376 (W/844318-844376) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 56, R 51, C 51 N 57. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO363555. SALKseq_126070.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G11080.2=P355 and AT5G11090.1=P355 (at chr 5 pos 3524010 (C/3524010-3524078) on the TAIR10). The sequences' 1-68 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 56, R 51, C 51 N 57. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO366902.
Donation Date: 2003-01-31
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_126070
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_126070C.
Status | Price (£) |
---|---|
Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
---|---|---|---|
|
|
|
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.