NASC

NASC ID: N626229

Name: SALK_126229

ABRC stock number: SALK_126229

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At3g62200 T17J13.160 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_126229.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G62200.1 (at chr 3 pos 23023627 (W/23023598-23023627) on the TAIR10). The sequences' 1-30 bps mapped on the genome, 41-79 bps onto the T-DNA. Pools P 54, R 57, C 60 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO360211.

SALKseq_126229.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G22426.1 and AT2G22430.1=UTR3 (at chr 2 pos 9522364 (C/9522364-9522407) on the TAIR10). The sequences' 1-44 bps mapped on the genome, 36-79 bps onto the T-DNA. Pools P 54, R 57, C 60 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO350185.

SALKseq_126229.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G43020.1 (at chr 3 pos 15055954 (W/15055902-15055954) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 54, R 57, C 60 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO353534.

SALKseq_126229.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G44840.1 (at chr 3 pos 16372914 (C/16372914-16372952) on the TAIR10). The sequences' 1-39 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools: P 54, R 57, C 60 N 53. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO356859.

Donation Date: 2003-01-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_126229

Part of : SALK lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G22426 AT2G22430 AT3G43020 AT3G44840 At3g62200

EMBL accession:

BZ354997

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.