NASC Stock Detail Page
NASC ID: N626569
Name: SALK_126569
ABRC stock number: SALK_126569
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. January 2003 TAIR gene annotation - At5g15270 F8M21.160 L300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_126569.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G15270.1 (at chr 5 pos 4958560 (C/4958560-4958635) on the TAIR10). The sequences' 1-76 bps mapped on the genome, 80-98 bps onto the T-DNA. Pools P 58, R 56, C 52 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO390362. SALKseq_126569.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G46875 and AT3G46880.1 (at chr 3 pos 17264799 (C/17264799-17264851) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 54-79 bps onto the T-DNA. Pools P 58, R 56, C 52 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO376980. SALKseq_126569.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G15160.2=UTR3 and AT4G15165.1=P355 (at chr 4 pos 8648240 (C/8648240-8648297) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 58, R 56, C 52 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO380327. SALKseq_126569.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G13180.1 (at chr 5 pos 4197888 (W/4197824-4197888) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 58, R 56, C 52 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO383672. SALKseq_126569.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G15240.1 (at chr 5 pos 4947517 (C/4947517-4947574) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 58, R 56, C 52 N 60. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO387015.
Donation Date: 2003-01-31
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_126569
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_126569C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.