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NASC ID: N626669

Name: SALK_126669

ABRC stock number: SALK_126669

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At3g22080 MZN24.29 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_126669.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G22080.1 (at chr 3 pos 7779626 (W/7779595-7779626) on the TAIR10). The sequences' 1-32 bps mapped on the genome, 53-79 bps onto the T-DNA. Pools P 56, R 58, C 54 N 54. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO370280.

SALKseq_126669.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G60900.1 (at chr 1 pos 22423880 (W/22423829-22423880) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 53-79 bps onto the T-DNA. Pools P 56, R 58, C 54 N 54. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO363586.

SALKseq_126669.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G27230.1 (at chr 4 pos 13638340 (C/13638340-13638389) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 51-77 bps onto the T-DNA. Pools P 56, R 58, C 54 N 54. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO373635.

SALKseq_126669.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G36340.1 (at chr 2 pos 15235694 (C/15235694-15235745) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 56, R 58, C 54 N 54. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO366936.

Donation Date: 2003-01-31

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_126669

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_126669C.

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
EMBL accession:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.