Home | About NASC | Address/Staff
Ask a question | Help

NASC

From January 1st, 2021, seed orders from EU customers will require a phytosanitary certificate. Please click for further information

NASC Stock Detail Page

NASC ID: N627176

Name: SALK_127176

ABRC stock number: SALK_127176

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At3g11010 F9F8.17 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_127176.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G11010.1 (at chr 3 pos 3453536 (W/3453460-3453536) on the TAIR10). The sequences' 1-77 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools P 57, R 53, C 51 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO407100.
SALKseq_127176.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G36080.2=P355 and AT2G36090.1=P355 (at chr 2 pos 15153244 (W/15153164-15153244) on the TAIR10). The sequences' 1-81 bps mapped on the genome, 75-95 bps onto the T-DNA. Pools P 57, R 53, C 51 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO403764.

Donation Date: 2003-01-31

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_127176

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_127176C.

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
EMBL accession:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.