NASC Stock Detail Page
NASC ID: N627405
Name: SALK_127405
ABRC stock number: SALK_127405
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. January 2003 TAIR gene annotation - At1g69030 T6L1.21 R300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_127405.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G69030.1 and AT1G69040.2=P355 (at chr 1 pos 25949455 (W/25949416-25949455) on the TAIR10). The sequences' 1-40 bps mapped on the genome, 37-60 bps onto the T-DNA. Pools P 55, R 60, C 53 N 52. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400438. SALKseq_127405.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G61795.1=UTR3 and AT1G61800.1=P355 (at chr 1 pos 22821566 (C/22821566-22821660) on the TAIR10). The sequences' 1-95 bps mapped on the genome. Pools P 55, R 60, C 53 N 52. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO397092. SALKseq_127405.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G72510.2=UTR3 and AT1G72520.1=P355 (at chr 1 pos 27305532 (W/27305473-27305532) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 55, R 60, C 53 N 52. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO403777. SALKseq_127405.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G59150.1=UTR3 and AT5G59160.2=P355 (at chr 5 pos 23878759 (W/23878701-23878759) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 55, R 60, C 53 N 52. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO407113.
Donation Date: 2003-01-31
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_127405
Part of : SALK lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.