NASC

NASC ID: N627932

Name: SALK_127932

ABRC stock number: SALK_127932

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At5g49190 K21P3.6 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_127932.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G49190.1 (at chr 5 pos 19946354 (C/19946354-19946413) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools P 59, R 58, C 56 N 56. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO383738.

SALKseq_127932.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G04625.1 (at chr 1 pos 1289732 (W/1289654-1289732) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 59, R 58, C 56 N 56. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO380396.

SALKseq_127932.101 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G12250.1 (at chr 5 pos 3963487 (C/3963487-3963550) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 59, R 58, C 56 N 56. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO387085.

Donation Date: 2003-01-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_127932

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_127932C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G04625 AT5G12250 At5g49190

EMBL accession:

BZ356016

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.